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Accurate diagnosis of tuberculosis (TB) is essential for the control of this infectious disease. The aim of the study was to assess the usefulness of non-tuberculous mycobacteria (NTM) growth mediums in the final identification of mycobacteria isolated from clinical samples and to compare the results with culture and DNA-fingerprinting methods. Mycobacteria were identified with the Bactec 12B and MGIT 960 BACTEC 13 and MGIT 960 TB (MGIT) automated liquid culture systems. TB cultures were subcultured onto Löwenstein-Jensen medium and MGIT was used as the primary culture system. The Bactec TB system was used with MGIT as the primary culture system. From January 1999 to December 2001, 230 mycobacteria were isolated from the respiratory tract (n=121), the pleura (n=35), the bone (n=28) and from other sites (n=40). Out of 230 mycobacteria, 188 were identified as Mycobacterium tuberculosis (Mtb) and 42 as nontuberculous mycobacteria (NTM) using the Bactec 13 system, 138 as Mtb and 31 as NTM using the 0b46394aab